Fusion of bacterial protoplasts.
نویسندگان
چکیده
منابع مشابه
Isolation and Fusion of Cotton Protoplasts
Protoplasts were isolated from five species of Gossypium. · Protoplast yield and viability were affected by incubation conditions, osmolarity, purification procedures, and cell source. Using an optimized procedure, highly viable protoplasts were isolated from cell suspensions, callus cultures, and leaf tissue of G. hirsutum, G. arboreum, G. k/otzschianum, G. harlmessii, and G. herbaceum. Protop...
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The electrical fusion technique of Zimmermann and Scheurich (1981 Planta 151: 26-32) has been used to fuse mesophyll protoplasts of Avena, Zea, Vigna, Petunia, and Amaranthus. Electrical fusion proves to be a simple, effective, and general fusion technique that can be controlled to form either dikaryons or large multinucleate fusion bodies. In addition, we show that Vigna mesophyll protoplasts ...
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Numerous morphological, physiological, and biochemical studies have been carried out on bacterial forms exhibiting a pronounced osmotic fragility (for review see Weibull, 1958b). Naked bacterial protoplasts, obtained by means of a complete removal of the cell wall by lysozyme in a sucrose medium (Weibull, 1953) offer an example of such an osmotically fragile structure. Osmotically sensitive for...
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Among protoplasts released from cells of Bacillus megaterium grown at 20, 30, or 37 C, osmotic swelling in NaCl solution at a given external osmotic pressure was greatest for protoplasts from cells grown at 20 C and least for protoplasts from cells grown at 37 C. Protoplasts from cells grown at lower temperaturs were also less stable to osmotic shock and lysed at higher external osmotic pressur...
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In a previous communication (I) it was reported that the cell walls of Streptococcus fecalis (ATCC 9790) were susceptible to digestion by lysozyme. This finding subsequently allowed the preparation of protoplasts from these organisms by treatment of whole cells with lysozyme and the use of high concentrations of sucrose as the impermeantl substance necessary for osmotic stabilization. Further i...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1976
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.73.6.2151